Anastasis in Glioblastoma, Brain Cancer Stem, and Brain Stem Cells

Objective: Anastasis, a process that has been discovered recently, halts apoptosis, and thus, recovers the survival functions of cells. It may be suggested anastasis is related to cancer progression, especially in stem cells which are responsible for therapy resistance, metastasis, recurrence. In this study, investigation phenomena its effect on stemness gene expressions brain cancer-related aimed. Methods: study commercially obtained glioblastoma multiforme(GBM), cells(BCSC), cells(BSC) were used vitro models. To induction apoptosis 4% ethanol-including medium was used. Annexin V assay confirmation apoptotic anastatic status. Gene expression profile determined real-time qRT-PCR method fold changes calculated by using 2-∆∆Ct method. Ingenuity Pathway Analysis functional pathway upstream regulatory analysis. Results: A common decrease genes GBM performed Although BSCs showed similar with cells, all upregulated BCSCs. Similar profile, canonical pathways markedly down-regulated BSC GBM, while they up-regulated BCSC. Differently, it activation self-renewal BCSC, unlike BSC. Conclusion: We have demonstrated inhibition prevent malignant transformation healthy aggression cancerous Anastasis as critical mechanism supports uncurable cancers.